![]() Additional information on the FACSCalibur and helpful links are included below. While I love the MoFlo and get excellent sorts from it, I do hate having to compensate 8. CytoML detects when a custom compensation matrix is used for analysis (e.g., in FlowJo) and. Samples should be provided in 12 x 75mm tubes at a concentration of approximately 1 x 10^6 cells/tube. Weve tried running them through FlowJo (and maybe Diva) with no luck. manual analyses performed in FlowJo, Diva, or Cytobank. Roan but training of individual users will be integrated into CBBC policy at a later date. Currently the FACSCalibur is run only by Ms. This brief guide is to help users new to compensation calculations and experienced flow-maestros alike breeze through this process in a painless and professional fashion. We strongly encourage anyone interested in utilizing this piece of equipment to contact Marilyn Dietrich to discuss your experiment before hand to ensure that the proper controls and fluorophores are used. The Compensation Wizard in FlowJo is one of the most frequently used platforms, and by extension potentially the greatest source of confusion on a per-cytometrist basis. Data collection and analysis is done with CellQuest Pro software and soon data can be analyzed on our new workstation with FlowJo software. In this webinar we'll discuss the use, benefits, and. ![]() The PBRC Equipment Committee has approved the addition of a FACSLoader to be installed on the FACSCalibur enabling automated measurements of 40 tubes to be done in sequence with minimal human input. Training Tutorials Reference Tools Reference Reading - Publishing Cytometry data & more about the technologies Manuals and User Guides Community. Compensation in FlowJo Jan 21 - YouTube The compensation options in cytometry have expanded significantly over the past few years. This instrument is equipped with two lasers (488nm and 635nm) and detectors for forward scatter, side scatter, and four fluorescence PMTs. The CBBC houses a BD FACSCalibur flow cytometer in room L4076. Intro FlowJo BASICS Aja Rieger 1.83K subscribers 9.7K views 1 year ago FlowJo Are you new to data analysis in FlowJo or looking for a starting point This video will give you a quick. Groups act like folders to organize your samples, allows master gating and unique report generation. PBRC Home :: CBBC Home :: Equipment :: FACSCalibur ![]()
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